ASSOCIATION OF ACTN3 R577X AND ACE I/D POLYMORPHISMS IN BRAZILIANS WRESTLERS

Marcelo Romanovitch Ribas,Julio Cesar Bassan,Zair Candido Oliveira Netto,Priscila Fernandes,Fabiano Salgueirosa,Oslei De Matos

doi:10.1590/1517-869220172306171864

Marcelo Romanovitch Ribas, Julio Cesar Bassan + Show 4 more

Open Access

https://doi.org/10.1590/1517-869220172306171864

Copy DOI

Abstract

ABSTRACT Introduction: By associating genetics and sport, it is possible to identify subjects with greater capacity to adapt to training, and lower chances of injury. Objective: The investigation evaluated the genotypic and allelic distribution of ACTN3 R577X and ACE I/D polymorphisms in Brazilian high-performance athletes in wrestling and percussion combat sports. Methods: The study included 37 male athletes ranked from first to third place in world scenarios, divided into two groups: wrestling (23 wrestlers, being 11 of Judo, 4 of Greco-Roman style, 8 of Brazilian Jiu Jitsu, with mean age of 27.3 ± 6.9 years) and percussion combat sports (14 athletes with a mean age of 25.7±4.4 years, being 6 of Karate, 3 of Muay Thai, 4 of Taekwondo, 1 Boxing). Genotyping of ACTN3 and ACE I/D polymorphisms was performed by polymerase chain reaction (PCR) from the genomic DNA. Genotypic and allelic distributions were compared with control populations and athletes by Chi-square test and Fisher’s exact test; all analyzes considered p ≤ 0.05. Results: The genotypic distributions and allelic frequencies of ACTN3 RR=46%, RX=38% and XX=16%; R=65% and X=35%, and ACE I/D DD=47.7%, ID=34.3% and II=20%; D=62.9% and I=37.1% did not differ from the control population; however, when compared with wrestling athletes a significant difference was observed. Conclusion: These results suggest an association of ACTN3 R577X and ACE I/D genes with Brazilian high-performance wrestling athletes.

Highlights

By associating genetics and sport, it is possible to identify subjects with greater capacity to adapt to training, and lower chances of injury
The genotypic frequency of ACTN3 R577X and ACE I/D polymorphisms between all of the wrestlers and the percussion group followed the Hardy-Weinberg equilibrim (ACTN3: x2=1.07 and 0.83, p=0.30 and 0.36, ACE: x2=2.47 and 0.03, p=0.11 and 0.85), but this did not occur with the domain group (ACTN3: x2 =4.15, p=0.04; CEA: x2=4.87, p=0.02)
There was no significant difference in genotypic distribution for the ACTN3 R577X polymorphism considering all wrestlers and when these were separate in grappling and percussion groups and the control Coelho[18] p≥0.05

Summary

Introduction

By associating genetics and sport, it is possible to identify subjects with greater capacity to adapt to training, and lower chances of injury. Objective: The investigation evaluated the genotypic and allelic distribution of ACTN3 R577X and ACE I/D polymorphisms in Brazilian high-performance athletes in wrestling and percussion combat sports. There is growing evidence that there are strong genetic influences on athletic performance and for an evolutionary change in performance characteristics for activities of speed, strength and resistance[13] This phenomenon can be illustrated by the variation R577X in ACTN3 gene, encoding the protein α-actinina-3, a protein that is necessary to perform explosive muscle actions. The objective of this research was to evaluate the genotypic and allele distribution of ACTN3 R577X and ACE I/D polymorphisms in Brazilian grappling and percussion wrestlers of high performance

Objectives

Methods

Results

Discussion

Conclusion