Abstract
Background: To investigate the microbiome composition in individuals with and without rosacea and correlate findings to individual factors that may affect facial cutaneous and enteric microbiome composition. Methods: Participants with and without rosacea (as determined by a board-certified dermatologist) were surveyed regarding factors that may affect the facial cutaneous/enteric microbiome. Microbiome samples were collected, analyzed for 16S sequences, and mapped to an optimized version of existing databases. R was used to perform Mann-Whitney/Kruskal-Wallis test for categorical comparisons. Correlation between two continuous variables was determined with linear regression models. Primary Component Analysis (PCoA) plots employed Monte Carlo permutation test to estimate p-values. All p-values are adjusted for multiple comparisons with the false discovery rate (FDR algorithm) using Benjamini-Hochberg. Results: 84 individuals with rosacea and 44 controls were evaluated. Individuals with rosacea were more likely to currently own pets (p = 0.029) and consume more alcohol (p = 0.006). Absolute bacteria abundance were similar in facial cutaneous (p = 0.36) and enteral microbiome (p = 0.29). Facial cutaneous microbiome showed significantly decreased richness and evenness (OTU: p = 0.019; Shannon: p = 0.049) and a three to four-fold decrease in abundance of 8 distinct cutaneous bacterial genera in rosacea. Enteral microbiome analysis showed significant reduction in abundance of Ruminococcaceae (FDR = 0.002) and Blautia (FDR < 0.001) and increase in Prevotellaceae (FDR = 0.024) in rosacea. Conclusion: Environmental factors may alter relative abundances of specific microbial genera and lead to microbiome diversity. Further studies with increased sample sizes and higher severity cases may further elucidate the role of dysbiosis in rosacea.
Highlights
Rosacea is a chronic inflammatory disorder of the central face, characterized by transient or persistent erythema and telangiectasias, papules and/or pustules, phymotic changes and/or rare ocular manifestations.[1]
More recent studies suggest that this inflammation may have a systemic component given rosacea’s association with various inflammatory conditions, including Crohn’s disease, ulcerative colitis, small intestinal bacterial overgrowth (SIBO), and Helicobacter pylori.[4,5,6,7,8]
Our findings demonstrated a correlation between rosacea and decreased diversity and relative of abundance of organisms within the facial cutaneous microbiome of individual’s with rosacea
Summary
Rosacea is a chronic inflammatory disorder of the central face, characterized by transient or persistent erythema and telangiectasias, papules and/or pustules, phymotic changes and/or rare ocular manifestations.[1]. The microbiome is a vast and varied collection of bacteria, viruses, and fungi whose composition has increasingly been demonstrated to have significant influence on whole-body health as well as development and maintenance of immunological activity.[1,9] Early exposure to commensal skin microbes and environmental factors affect the developing microbiome’s richness (i.e., diversity of organisms) and evenness (i.e., relative quantity of organisms present) and may influence the function of the immune system and inflammatory response.[9,10,11] Studies have found a correlation between the relative abundances of several cutaneous microbes, including notably Demodex folliculorum (and its native microbe Bacillus oleronius), virulent strains of Staphylococcus epidermidis, cytotoxin-associated gene A positive (CagA+) Helicobacter pylori and Chlamydophila pneumoniae, and rosacea.[1,12,13,14,15] diseases and isolate and analyze the impact of environmental factors. To investigate the microbiome composition in individuals with and without rosacea and correlate findings to individual factors that may affect facial cutaneous and enteric microbiome composition
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call