Abstract
Background: Endothelin-1 plays a role as a vasoconstrictor and also inhibits the expression of adiponectin in obesity. The Lys198Asn polymorphism of the endothelin-1 gene will increase the level of endothelin-1 plasma and increase the risk factor of obesity.
 Objective: In this study, we investigated the relationship between Lys198Asn polymorphism of endothelin-1 gene and endothelin-1 plasma level among individuals with obesity in Javanese population.
 Method: This study was conducted on 61 obese subjects and 65 control subjects. All subjects were measured for anthropometrics and endothelin-1 plasma level by ELISA (Enzyme-linked Immune Sorbent Assay). The polymorphism of Lys198Asn of ET-1 (Endothelin-1) was screened by using PCRRFLP (Polymerase Chain Reaction- Restriction Fragment Length Polymorphism).
 Results: TT genotype was a risk factor of obesity (OR 5.344 CI=95% 1.77-16.16) compared with GG genotype. Levels of endothelin-1 plasma were higher in obese subjects than that of control subjects (p=0.013), with TT genotype having the highest endothelin-1 plasma level (p<0.001).
 Conclusion: The Lys198Asn polymorphism of endothelin-1 gene plays a role in increasing endothelin-1 plasma levels and is risk factors of obesity.
 Bangladesh Journal of Medical Science Vol.18(1) 2019 p.46-49
Highlights
Obesity is defined as a state with excessive fat accumulation in adipose tissue inside the body as a result of imbalance between energy intake and activity[1]
The Lys198Asn polymorphism of endothelin-1 gene plays a role in increasing endothelin-1 plasma levels and is risk factors of obesity
The gene involved in coding endothelin-1 is 5.5 kb long located at chromosome 6 and consists of 5 exons and 4 introns and is the strongest vasoconstrictor in human[4]
Summary
Obesity is defined as a state with excessive fat accumulation in adipose tissue inside the body as a result of imbalance between energy intake and activity[1]. The increase of endothelin-1 levels has been related with hypertension, stroke, diabetes mellitus[6], and obesity through inhibition of adiponectin production[7]. Lys198Asn polymorphism, is related with increased level of endothelin-1 plasma. This polymorphism is often studied for its relationship with hypertension[8] and stroke, but few studies examined it as a risk factor of obesity. The Lys198Asn polymorphism of the endothelin-1 gene will increase the level of endothelin-1 plasma and increase the risk factor of obesity. Objective: In this study, we investigated the relationship between Lys198Asn polymorphism of endothelin-1 gene and endothelin-1 plasma level among individuals with obesity in Javanese population. The polymorphism of Lys198Asn of ET-1 (Endothelin-1) was screened by using PCR-
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