Association between HOTAIR Polymorphisms and Lymphoma.

Sara Kashani,Gholamreza Bahari,Behrouz Mollashahi,Seyed Mehdi Hashemi,Hosein Ali Sasan,Mohsen Taheri

doi:10.31557/apjcp.2021.22.9.2831

Abstract

Introduction:The long non-coding RNA, HOTAIR, involved in cancer initiation and development. Objective:The aim of present study was to investigate the association of single nucleotide polymorphisms in the HOTAIR gene with lymphoma. Methods:We conducted a case-control study of 156 individuals with non-Hodgkin Lymphoma (NHL), 53 individuals with Hodgkin’s lymphoma (HL), and 245 unrelated healthy individuals to identify the genotype frequencies of each polymorphism. Genotyping of the SNPs (rs920778 T>C, rs1899663 G>T, rs4759314 A>G and rs12826786 C>T) was carried out using the polymerase chain reaction-restriction fragment length polymorphism. Results and Conclusion:The finding showed that rs1899663 variant of HOTAIR gene significantly decreased the risk of NHL in codominant, dominant, over-dominant and allelic inheritance models. We did not find any association between HOTAIR rs12826786, rs920788 and rs4759314 variants and NHL. The results indicated that neither the overall chi-square comparison of the cases and controls, nor the logistic regression analysis showed any association between HOTAIR polymorphisms and HL. Conclusively, our findings showed that rs1899663 of HOTAIR significantly decreased the risk of non-Hodgkin Lymphoma.

Highlights

The long non-coding RNA, HOTAIR, involved in cancer initiation and development
The finding revealed that rs1899663 variant of HOTAIR gene significantly decreased the risk of non-Hodgkin Lymphoma (NHL) in codominant (OR = 0.22, 95% confidence intervals (CIs) = 0.13–0.36, p < 0.001, GT vs GG), dominant (OR = 0.25, 95% CI = 0.15–0.41, p < 0.0001, GT+TT vs GG), overdominant (OR = 0.30, 95% CI = 0.19– 0.46, p < 0.0001, GT vs GG+TT) and allelic (OR = 0.66, 95% CI = 0.49–0.88, p =0.005, T vs G) inheritance models
The results demonstrated that HOTAIR rs12826786, rs920788 and rs4759314 variants were not associated with risk of/protection from NHL in codominant, dominant and recessive tested inheritance models (Table 2)

Summary

Introduction

The long non-coding RNA, HOTAIR, involved in cancer initiation and development. Objective: The aim of present study was to investigate the association of single nucleotide polymorphisms in the HOTAIR gene with lymphoma. Methods: We conducted a case-control study of 156 individuals with non-Hodgkin Lymphoma (NHL), 53 individuals with Hodgkin’s lymphoma (HL), and 245 unrelated healthy individuals to identify the genotype frequencies of each polymorphism. Results and Conclusion: The finding showed that rs1899663 variant of HOTAIR gene significantly decreased the risk of NHL in codominant, dominant, over-dominant and allelic inheritance models. The results indicated that neither the overall chi-square comparison of the cases and controls, nor the logistic regression analysis showed any association between HOTAIR polymorphisms and HL. Our findings showed that rs1899663 of HOTAIR significantly decreased the risk of non-Hodgkin Lymphoma. Environments as well as genetic factors are two major risk factors for lymphoma (Zhong et al, 2019; Tang et al, 2020)

Objectives

Methods

Results

Conclusion