Association between FNDC5 gene polymorphism and carotid atherosclerosis in patients with type 2 diabetes mellitus

Abstract

Objective To investigate the relationship between fibronectin type Ⅲ domain-containing protein 5 (FNDC5) gene polymorphism and carotid atherosclerosis (CAS) in patients with type 2 diabetes mellitus (T2DM). Methods A total of 358 patients with T2DM who were hospitalized in the Department of Geriatrics in Yan′an People′s Hospital from January 2017 to July 2018 were recruited in this study (T2DM group). These patients were divided into non-CAS group and CAS group according to the results of carotid ultrasonography. Meanwhile, 200 healthy volunteers were selected as control group. Genotype was determined by matrix assisted laser desorption/ionization time-of-flight mass spectrometry for the rs16835198, rs3480 and rs1570569 polymorphism of FNDC5 gene in all subjects. Results Compared with control group, there was no significant difference in genotype and allele frequencies of rs16835198, rs3480 and rs1570569 in T2DM group (χ2=0.279-2.582, all P>0.05). The GG genotype and G allele frequencies of rs16835198 in CAS group were significantly higher than those in non-CAS group (χ2=14.129, P=0.001; χ2=14.177, P=0.000). The T allele frequency of rs1570569 in CAS group was also significantly higher than that in non-CAS group (χ2=4.423, P=0.035). The results of logistic regression analysis showed that G allele of rs16835198 was risk factor for CAS in patients with T2DM (OR=1.786, 95%CI: 1.136-2.809, P=0.001). Conclusion The rs16835198 polymorphism of FNDC5 gene may be related to the susceptibility of CAS for patients with T2DM. Key words: Fibronectin type Ⅲ domain-containing protein 5; Irisin; Carotid atherosclerosis; Type 2 diabetes mellitus; Gene polymorphism