Abstract
Background: GnRH-1 mainly exists in hypothalamus and it is vital to gonad development and sexual maturity of animals. This study mainly analyzed the association between GnRH-1 polymorphism and carcass traits of quails, Research conclusions can provide references for breeding of new species of quail.Methods: From 2018 to 2019, 31 female samples of Chinese Yellow quail, beijingbai quail and Korean quail were collected in Luolong District, Luoyang City, Henan Province, China.SNP mutation sites of GnRH-1 gene were investigated in China yellow quail, Beijing white quail and Korean quail through PCR amplification and DNA sequencing technologys. Moreover, correlation analysis between polymorphism sites of GnRH-1 gene and carcass traits of quail was performed. Result: According to research results, a total of 14 SNP mutation sites of GnRH-1 were detected in China yellow quail, Beijing white quail and Korean quail, which were C71T, C108T, C168T, C178T, A184G, C206T, A209C, C215T, A252G, A279T, C281T, C293G, C339T and C458T. For A209C and C281T, only 2 genotypes were detected in China yellow quail and Beijing white quail, while for the remaining 12 SNP mutation sites, 3 genotypes were detected in three quail species. Correlation analysis showed that A209C, A279T, C281T and C339T were significantly correlated with liver weight (P less than 0.05), A209C, C281T and C339T were significantly correlated with dressing percentage (P less than 0.05), C215T and A279T were significantly correlated with breast muscle weight(whole) (P less than 0.05), A209C was significantly correlated with body weight, dressed carcass weight and whole net carcass weight (P less than 0.05).
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