Abstract
We have studied [ 3H]-dopamine ([ 3H]-DA) release from rat nucleus accumbens lateral septum slices in response to various paradigms aimed at increasing endogenous or exogenous thyrotropin releasing hormone (TRH) concentrations in the extracellular space. High KCl concentrations significantly enhanced [ 3H]-DA release by fourfold. TRH (10 −4 or 5 × 10 −4 M) did not affect [ 3H]-DA release. The release of [ 3H]-DA was not stimulated by TRH either in the presence of N-l-carboxy-2-phenylethyl (N imbenzyl)-histidylβnaphthylamide, a specific pyroglutamyl peptidase II inhibitor, or that of specific inhibitors of prolyl endopeptidase and pyroglutamyl peptidase I. None of the peptidase inhibitors modified the [ 3H]-DA release by themselves. These results suggest that the TRH stimulation of [ 3H]-DA release in vitro observed in previous studies is not due to peptide inactivation but may be due to a nonspecific effect. TRH enhancement of DA release in nucleus accumbens in vivo may not be the result of a direct effect of TRH on DA terminals.
Published Version
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