Assessment of salivary Interleukin (IL)-6, IL-10, Oxidative Stress, Antioxidant Status, pH, and Flow Rate in Dental Caries Experience patients in Tikrit Province

Abstract

Background: Dental caries is an infectious and communicable disease and multiple factors influence the initiation and progression of the disease. Imbalance between oxidative stress and saliva antioxidants plays a major role in initiation and spread of dental caries. Objective: The aim of this study was to evaluate physicochemical properties of saliva such as flow rate, pH, buffering capacity, interleukin(IL)-6, IL-10, and malondialdehyde (MDA),and total antioxidants capacity (TAC), and catalase (CAT) levels in caries free and caries active individuals. Material and Method: Fifty consecutive subjects with dental caries experience (27 M & 23F) and fifty caries free subjects (24 M & 26 F) were recruited. The dental caries status was assessed depending on the WHO oral assessment form. Stimulated saliva samples were collected at the morning from both groups. The MDA, TAC, and CAT were evaluated using spectrophotometric assay. SPSS 13 analyzed data using Student’s t-test. Results: There was a statistically significant increase in salivary T A C, CAT, and IL-6 in group with dental caries compared with control group. However, the salivary pH, flow rate, buffering capacity, and IL-10 levels were statistically lower in caries active patients. Person's correlation coefficient indicated significant negative correlations between the IL-6 with IL-10, pH, MDA, and flow rate. Whereas positive correlation with TAC, and buffering capacity. In addition, there was negative correlation between IL-10 with TAC, MDA, and buffering capacity, While positive correlation with pH and Flow rate. Conclusions: Alterations in salivary MDA, TAC, CAT, and IL-6 levels were significantly higher whereas Salivary pH, flow rate, buffering capacity, and IL-10 were significantly lower in the study groups when compared to control groups.