Assessment of Potassium Bromate, Calcium Sulfate and Synergistic Toxic Effects in Male Mice

Abstract

Food additives are the basis of the modern food industry, and play an important role in improving the color, smell, and taste of food, altering its nutritional structure, perfecting its processing conditions, and extending its shelf life. Twenty four adult male Swiss albino mice were used in our experiment : Group (1) mice were Intraperitoneally injected of normal saline 0.5 ml (as control). Group (2) mice were intraperitoneally injected of well dispersed Potassium Bromate 320 mg/kg of body weight for two months (twice weekly). Group (3) mice were faded Calcium sulfate as chew of food 0.03% w/w for two months (daily intake). Group (4) mice were intraperitoneally injected of well dispersed Potassium Bromate 320 mg/kg of body weight for two months (twice weekly) and also given Calcium sulfate as chew of food 0.03% W/W for two months (daily intake). After the end of project protocol the mice had been sacrificed and blood collocated and this parameters was evaluated white blood cells (WBC) , red blood cells (RBC) ,platelet (PLT),hematocrit (HCT), and hemoglobin concentration (Hgb). The result showed there is highly significant decrease for WBC for the (group 2 and group 4) , Red blood cell count show that there is highly significant decrease of (group 2 and4), In contrast the result for Hemoglobin concentration show there were highly significant increase in group 2 and 4 by comparing with control group , also highly significant increase for Hct percentage for the (group 2 and 4) by comparing with control group and group 3. Finally platelets count result show that there is highly significant decrease in group 2 and group 4. From current study We can conclude that there are different harmfully effects of KBrO3 and the synergistic effect with CaSo4 on the animal model and according to that on human due to the daily uptake of this compounds in our life style ,according to the hematological indices (WBC),(RBC), (Hgb), (Hct), and finally (PLT). Thus the using of KBrO3 and CaSo4 should be limited and used specific documented concentration to reduce its unfavorable effects