Assessment of phytochemical screening by Fourier Transform Infrared spectroscopic analysis of peach (Prunus persica) seed biomass from Uttarakhand region of India

Abstract

Prunus persica (Peach) has several medicinal and nutritive properties such as antioxidant, antimalarial, anticoagulant, antifungal, ant-allergic, etc. The present study focuses on the exploratory for phytochemicals constituents screening of seed extracts of Prunus persica from Uttarakhand region of India by Fourier Transform Infrared spectroscopy (FTIR) analysis. The extraction of seed was carried out using soxhlet apparatus in different solvents such as petroleum ether, chloroform, ethyl acetate, ethanol, and water. The characteristics of physical parameters of dried seed of P. persica were reported as total ash (14.250±0.126 %), acid insoluble ash (6.800±0.036 %), water-soluble ash (11.714±0.123 %), and sulphated ash value (2.274±0.025 %); whereas, the extractive values were also recorded as alcohol soluble extractive (1.917±0.011 %), and water-soluble extractive (10.580±0.048 %). The highest values of nutritive components (366.778±0.257 %) were followed by, carbohydrate (45.500±0.336 %), crude protein (29.360±0.551 %), available carbohydrate content (28.002±0.439 %), moisture content (12.547±0.022 %), crude fibre (11.602±0.123 %), crude fat (7.482±0.068 %), and total nitrogen (4.695±0.032 %). The maximum extraction yield was recorded in the aqueous extract solution (11.15 %), followed by petroleum ether (2.8 %), ethyl acetate (2.1 %), ethanol (1.6 %), and chloroform (0.4 %). Besides, this the most effective chemical bonding groups of FTIR spectra analyzed in a sample of P. persica seed were N-H, O-H (3600-3400 1/cm), C-H (3000-2800 1/cm), N-H, C-C, C=O (1680-1550 1/cm), C-H (1470-1350 1/cm), CO-OR (1400-1000 1/cm), C-H (850-550 1/cm), and C-I (500-400 1/cm), respectively. Therefore, this study provides useful insights into the beneficial properties of P. persica seed biomass from the Uttarakhand region of India, which may be further used for the production of several pharmaceuticals and nutraceutical products.