Assessment of oxidative proteins modification level in infertile males ejaculate secondary to toxocara invasion

Abstract

Our research was aimed at revealing level of proteins oxidative proteins modification in ejaculate homogenate for patients with reproductive damages secondary to toxocara invasion.
 Materials and methods. The article contains the data of researching proteins oxidative modification in ejaculate and values for congenital immunity cells factors in 89 men being divided into five groups depending on spermatozoa DNA values and presence or lack of toxocara invasion.
 The results. According to the data obtained it has been established that increasing OPM values with different intensity in ejaculate depending on peculiarities of damaging factor were being observed. Incomplete phagocytosis of the neutrophil link was determined, but only in the 3rd and 4th groups was noted a simultaneous decrease in HSTsp and HSTst, which indicated a deep damage to the bactericidal system of phagocytes, which also, apparently, occurs due to inhibition of the metabolism of immunocompetent cells and their bioenergetic resources by toxic products of life activity of helminths. It was in the groups with the most pronounced oxidative stress that DNA fragmentation of spermatozoa was also observed, which, in turn, was combined with the more severe forms of pathozoospermia in the 4th and 5th groups we discovered earlier.
 Conclusions. Thus, insignificant OPM increasing in patients of the third and fourth groups (with antibodies against toxocara) is connected with defense formed by helminths against damaging factors, such as OPM and phagocytosis, that is confirmed by incomplete phagocytosis secondary to functional and metabolic reserve deficiency. High OPM values in patients of second and fifth groups indicate only influence of environment (industrial pollutants in air), many of them can be themselves booster to develop and maintain OPM high values.