Assessment of ionic homeostasis imbalance and cytochrome P450 system disturbance in mice during fumonisin B1 (FB1) exposure

Abstract

Fumonisin B1 (FB1) is a mycotoxin frequently found in agricultural commodities, and poses a considerable risk for human and animal health. The aim of this study was to investigate the toxic effect of FB1 in mice intestine. Male Kunming mice (n = 40) were treated with FB1 diet for 42 days. Histopathological and biochemical analyses, including ion concentrations, transcription of ATPase subunits and mRNA expression of cytochrome P450s (CYP450s) analyses were performed on duodenum, cecum and colon of mice. The results revealed that FB1 caused histological alterations, including partial shedding of villous epithelial cells and inflammatory cell infiltration. Furthermore, a significant change in Na+, K+ and Ca2+ in serum, and the mRNA expression of ATPase subunits and CYP450s in intestinal tracts were observed in FB1-exposed mice. Our results suggested that FB1 exposure induce histopathological injury via disrupting CYP isoforms transcription and triggering ion homeostasis imbalance in mice intestinal tracts.