Assessment of Genetic Purity of Parental Lines of Hybrid Rice Using DNA-Based Markers

Sunil Bhavsar,Neeru Jain,Twinkle Solanki,Suchita Amin

doi:10.3844/ojbsci.2015.59.69

Abstract

Assessment of hybrid seed genetic purity is one of the most important quality control parameters in hybrid seed production. With the objective of replacing the GOT with DNA based assays, Cytoplasmic Male Sterile (CMS), restorer and hybrid lines have been screened by means of microsatellite and Sequence Tagged Site (STS) markers. Cytoplasmic Male Sterile (CMS) lines often get contaminated with cognate isonuclear maintainer lines during multiplication. The PCR assay was able to detect precisely the impurities in a commercial seed lot of CMS line. The extent of heterozygosity within parental lines of rice hybrids were assessed and the results suggest that a single, appropriately chosen microsatellite marker should be sufficient for assessing hybrid seed purity.

Highlights

Rice, Oryza sativa L. is the most important staple crop feeding over half of the world population
As reported in literature four Cytoplasmic Male Sterile (CMS) lines viz., IR 58025, IR 68897, IR 79128, IR 79156 generated a band of 386 bp in CMS lines, which was absent in their counter maintainer lines (Fig. 2 A, B and C)
It was concluded that CMS lines have the band, but it is completely absent in maintainer lines as well as all restorer

Summary

Introduction

Oryza sativa L. is the most important staple crop feeding over half of the world population. The present world population of about 6 billion is likely to cross 8 billion by the year 2030, which necessitates that rice production must increase by 40% to keep pace with the increasing trend in the population growth. Hybrid rice is one of the technological options that will impact the worlds rice production (Khush, 2005). Hybrid rice technology appears to be a feasible and readily available option for raising yield potential. China, hybrid rice is successfully grown in countries like India, Vietnam, Philippines, Latin American countries, to a small extent only. Though the area occupied by hybrid rice in India is still small but it is expected to increase substantially

Methods

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Conclusion