Abstract
Genetic diversity was evaluated among 14 cultivars of Catharanthus roseus using RAPD and ISSR markers. The RAPD primers resulted in the amplification of 56 bands, among which 46 (82%) bands were polymorphic Four ISSR primers amplified 31 loci out of which 17 were polymorphic and 14 are monomorphic. The Jaccard's similarity derived from the combined marker system showed that the varieties First Kiss Coral and Cooler Orchid were the most closely related cultivars, with 98% similarity. In the dendrogram constructed on the basis of both RAPD and ISSR data two clear clusters were obtained. The smaller cluster included C. roseus Cv Blue Pearl and C. roseus Cv. Patricia White and the larger cluster was subdivided into two sub clusters with C. roseus Cv. First Kiss Polka Dot isolated from the rest of the cultivars. This may be useful for breeding for improved quality.
Highlights
The resolving power of the random-amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSRs) primer was calculated according to the method of Prevost and Wilkinson (1999)
25 random decamer oligonucleotide primers were used for fingerprinting of 14 Catharanthus roseus cultivars
All these 14 primers resulted in the amplification of 56 bands, of which 46 bands (82%) were polymorphic while only 10 bands (18%) were monomorphic, indicating the presence of a high degree of genetic variation in the studied cultivars
Summary
New and improved varieties of Vinca are available. Catharanthus roseus (Vinca rosea) is native to Madagascar and spread throughout the tropics and subtropics. Though considerable variation can be observed in gardens around the world, no information on the origin is available not even attempts have been made so far to study the genomic relations among Vinca. The polymerase chain reaction (PCR) based on molecular markers such as random-amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSRs) are being extensively used to study the genetic diversity in a number of plant species at the species as well as cultivar level. No report is available on applications of molecular markers in studies on the genetic diversity of different Catharanthus roseus
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