Abstract
Dual-wavelength UV detection is widely employed to generate absorbance ratios as a relatively non-specific method for characterizing peak purity in liquid chromatography. The sensitivity of this method is largely dependent on the pair of wavelengths chosen. An alternative technique, correlation of the spectral information, proposed primarily to overcome this constraint, has been investigated. The implications for the application of correlation coefficients to peak purity and homogeneity determination are examined. Particular attention is focused on the successful use of correlating multiple chromatographic peak-area data (peak areas calculated from the absorbance values at various detection wavelengths) for the reliable assessment of both peak identity and peak homogeneity. Using a model system, 1% of a putative related compound of sulphasalazine in a mixture with sulphasalazine could be detected regardless of the chromatographic resolution between the two compounds.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
