Assessment Of Biofilm Production Of Obligatory Aerobic And Anaerobic Bacteria For Acne Patients In Kirkuk – Iraq

Abstract

The study which involved 200 patients with Acne vulgaris, was carried out in Kirkuk City from October 2021 to March 2022. Toestablish the bacterial species that create a biofilm and to isolate and identify the bacterial species that causes Acne vulgaris patients.Acne swab were obtained for aerobic and anaerobic culture, microtiter plate method (MPA), and congo red agar (CRA), methods wereused to detect the biofilm formation.Propionibacterium acnes plays a role in the pathogenesis of acne vulgaris, a common disorder of the pilosebaceous follicles and it hasbeen suggested that P. acnes cells residing within the follicles grow as a biofilm.The most common isolated bacteria P.acne 40.5%, followed by S.epidermidis 22.5%, S.aureus 19.5%, and 7% S.pyogenes . The resultsof the current study indicate that type of bactera isolates were strong biofilm producer by microtiter plate method (MPA) and congored agar method , 88.9% of P.acne,73.3% of S. epidermidis , 64.1% of S. aureus , and 70% of S.pyogenes , isolates were strongbiofilm producer by MPA, while the results also showed the74.0% of P.acne ,73.3% of S. epidermidis , 71.7% of S. aureus ,70% ofS.pyogenes isolates were strong biofilm producer by CRA