Assessment of antithyroid activity of 2,8-disulfanyl-1,9-dihydro-6H-purin-6-one in vitro and in vivo

Abstract

2,8-Disulfanyl-1,9-dihydro-6H-purin-6-one has been examined in vitro and in vivo, for its potential antithyroid effects. It exhibited stable higher order complexation with iodine in vitro, which is a sign of its possible antithyroid activity. The blood assays of rats were carried out using radioimmunoassay technique to determine free triiodothyronine and thyroxin levels and with ELISA method for thyroid stimulating hormone concentration; which showed a decrease in the former two hormone levels and increase in the latter, for the animals treated with 2,8-disulfanyl-1,9-dihydro-6H-purin-6-one as compared to the control groups. Similarly, thyroid histology of the treated animals depicted hyperactive gland conditions with little colloid material and cylindrical shape of follicular epithelium as compared to the normal cuboidal epithelium with sufficient stores of colloid material in the control animals, which confirms the antithyroid activity of the 2,8-disulfanyl-1,9-dihydro-6H-purin-6-one. The results were also compared with the animals treated with methimazole (1-methyl-1H-imidazole-2-thiol), the well known antithyroid drug and it was found that 2,8-disulfanyl-1,9-dihydro-6H-purin-6-one is more potent than methimazole as far as hormonal variations are concerned.