Abstract
BackgroundTo detect carbapenemase-producing Gram-negative bacteria in bacterial laboratories at medical settings, a new immunochromatographic assay for New Delhi metallo-β-lactamases (NDMs) was developed.MethodsThe immunochromatographic assay for New Delhi metallo-β-lactamases producers was developed using rat monoclonal antibodies against NDMs. The assessment was performed using 350 isolates of Gram-negative bacteria, including Acinetobacter baumannii (51 isolates), Enterobacteriaceae (163 isolates), and Pseudomonas aeruginosa (136 isolates) obtained from 2015 to 2017 in medical settings in Myanmar. Of them, 302 isolates were resistant to carbapenems, including imipenem and/or meropenem. The blaNDM genes were identified by PCR and sequencing.ResultsOf the 350 clinical isolates tested, 164 (46.9%) (60 isolates of Escherichia coli, 51 isolates of Klebsiella pneumoniae, 25 isolates of Enterobacter cloacae, 23 isolates of P. aeruginosa, and 5 isolates of A. baumannii) were positive on this assay, and all the positive isolates harbored genes encoding NDM-1, − 4, − 5 and − 7. The remaining 186 (53.1%) isolates negative on the assay did not harbor genes encoding NDMs. The assay had a specificity of 100% and a sensitivity of 100%. The assessment revealed that more than 90% of carbapenem-resistant Enterobacteriaceae produced NDMs.ConclusionsThe immunochromatographic assay is an easy-to-use and reliable kit for detection of NDMs-producing Gram-negative bacteria. The assay revealed that NDM-producing Enterobacteriaceae isolates are wide-spread in medical settings in Myanmar.
Highlights
To detect carbapenemase-producing Gram-negative bacteria in bacterial laboratories at medical settings, a new immunochromatographic assay for New Delhi metallo-β-lactamases (NDMs) was developed
Enterobacteriaceae, including Citrobacter freundii, Enterobacter spp., E. coli and K. pneumoniae, producing New Delhi metallo-β-lactamase-1 (NDM-1), NDM-4, NDM-5 or NDM-7 were isolated in medical settings in Myanmar [6,7,8,9]
Bacterial strains Strains assayed in this study included 51 isolates of A. baumannii, 27 of Enterobacter cloacae, 77 of E. coli, 59 of K. pneumoniae and 136 of P. aeruginosa obtained from individual patients in medical settings in Myanmar
Summary
To detect carbapenemase-producing Gram-negative bacteria in bacterial laboratories at medical settings, a new immunochromatographic assay for New Delhi metallo-β-lactamases (NDMs) was developed. Metallo-β-lactamases (MBLs) are produced by many species of Gram-negative bacteria, as well as some species of Gram-positive bacteria, including Bacillus spp. New Delhi metallo-β-lactamase-1 (NDM-1) was initially detected from strains Klebsiella pneumoniae and Escherichia. Enterobacteriaceae, including Citrobacter freundii, Enterobacter spp., E. coli and K. pneumoniae, producing NDM-1, NDM-4, NDM-5 or NDM-7 were isolated in medical settings in Myanmar [6,7,8,9]. To detect carbapenemase-producing Gram-negative bacteria in bacterial laboratories at medical settings in Myanmar, a. Tada et al BMC Infectious Diseases (2019) 19:565 new immunochromatographic assay for NDMs was developed and evaluated using Gram-negative bacteria in medical settings in Myanmar
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