Abstract
ABSTRACT Background: 25-hydroxyvitamin D-1-α-hydroxylase is a key enzyme for conversion of 25-(OH)-D to 1,25-(OH)2-D and is extra renally produced in various tissues. The objective of the study was to determine the levels of enzyme 1-α-hydroxylase in response to supplementation with vitamin D in premenopausal women. Methods: This single-arm pre-post interventional study, included premenopausal females (n=82) and their 1-α hydroxylase levels were measured from peripheral blood. Vitamin D supplements were given and vitamin D levels and α-1 hydroxylase levels were measured through ELISA. The serum vitamin D levels below 30ng/ml (75nmol/L), were considered vitamin D deficient. The correlation between serum vitamin D levels and 1-α-OHase levels was determined using Spearman’s Rank Correlation test, Wessa P Spearman Rank Correlation in Free Statistics Software. Relationships between variables were done through paired t-test and p<0.05 was considered significant. Results: The levels of 1-α (OH)ase varied in response to supplementation, with most values observed between 10-20ng/ml. The average increase in serum vitamin D and 1-α-(OH)ase levels was 9.49ng/mL and 4.66ng/mL respectively. In total 50(60.9%) samples revealed a decrease in 1-α (OH)ase levels, and 32(39.1%) samples increased. Data analysis showed t (84) = 1.214, p=0.228 for serum 1- α (OH)ase. For post-supplementation the results showed no association between (rs = 0.04982, p = 0.65671) the two variables. Conclusion: Serum levels of 1-α-hydroxylase in premenopausal Pakistani females indicated variation when vitamin D was supplemented, highlighting a non-linear relationship. This shows involvement of other unknown factors in Vitamin D metabolism, which requires to be investigated in future research. Keywords: Biopsy; Inflammation; Hydroxylase.
Highlights
Research on vitamin D and its derivatives has reached its zenith in the past few years
Serum levels of 1-α-hydroxylase in premenopausal Pakistani females indicated variation when vitamin D was supplemented, highlighting a non-linear relationship. This shows involvement of other unknown factors in Vitamin D metabolism, which requires to be investigated in future research
This effect is protective from the standpoint of skin cancer prevention since it reduces the skin’s ability to absorb ultraviolet B waves (UVB), but the other side of the coin is a decrease in Vitamin D production[2,3]
Summary
Research on vitamin D and its derivatives has reached its zenith in the past few years. There is a higher preponderance in countries that lie on or near the equator, as countries receiving stronger ultraviolet B waves (UVB) throughout the year are home to individuals with more melanin. This effect is protective from the standpoint of skin cancer prevention since it reduces the skin’s ability to absorb UVB, but the other side of the coin is a decrease in Vitamin D production[2,3]. Once-a-day dosing of 40 micrograms of calcidiol has been suggested[1]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
