Abstract
Lipid-based nanoparticles (LNPs) are widely used for the delivery of drugs and nucleicacids. Although most of them are considered safe, there is confusing evidencein the literature regarding their potential cellular toxicities. Moreover, little isknown about the recovery process cells undergo after a cytotoxic insult. We havepreviously studied the systemic effects of common LNPs with different surfacecharge (cationic, anionic, neutral) and revealed that positively charged LNPs ((+)LNPs) activate pro-inflammatory cytokines and induce interferon response by actingas an agonist of Toll-like receptor 4 on immune cells. In this study, we focusedon the response of human fibroblasts exposed to LNPs and their cellularrecovery process. To this end, we used image-based high content analysis(HCA). Using this strategy, we were able to show simultaneously, in severalintracellular parameters, that fibroblasts can recover from the cytotoxic effects of(+)LNPs. The use of HCA opens new avenues in understanding cellular response and nanotoxicityand may become a valuable tool for screening safe materials for drug delivery and tissueengineering.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call