Abstract
Twelve cDNA libraries from two species of catfish have been sequenced, resulting in the generation of nearly 500,000 ESTs.
Highlights
Through the Community Sequencing Program, a catfish expressed sequence tag (EST) sequencing project was carried out through a collaboration between the catfish research community and the Department of Energy’s Joint Genome Institute
Results cDNA libraries and EST sequencing Twelve cDNA libraries were constructed from various tissues, organs, and cell lines, including four blue catfish libraries and eight channel catfish libraries (Table 1)
A total of 438,321 ESTs were generated from this project, of which 128,711 sequences were from blue catfish and 309,610 were from channel catfish (Table 1)
Summary
While channel catfish (Ictalurus punctatus) accounts for the majority of commercial aquaculture production, the closely related blue catfish (Ictalurus furcatus) possesses several economically important traits that led to the production of an inter-specific hybrid (channel catfish female × blue catfish male) available for commercial use [1]. Large numbers of ESTs have been produced for most model species as well as a number of agriculturally important species [27,28,29,30,31,32], including cattle (1.5 million), swine (1.4 million), chicken (600,000), Atlantic salmon (471,000), and rainbow trout (281,000) The availability of such EST resources has allowed efficient gene discovery and gene identification in these species, and rapid progress has been made through comparative genome analysis in understanding structural, organizational, and functional properties of the genomes of these species
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