Abstract
Higher plants, algae and cyanophytes contain two molecules of chlorophyll a′ per reaction centre (RC) I, and only one is extracted with chloroform. In contrast, two molecules of phenophytin a are associated with RC II. Therefore the RCs can be assayed by simple high performance liquid chromatography (HPLC) quantitation of these pigments. In view of this, the process of chromatic regulation in two cyanophyte species ( Synechocystis PCC 6714 and Anacystis nidulans R 2, in which the RC I to RC II molar ratio changes significantly with a change in light quality) has been studied by chloroform extraction and HPLC. The results agree well with the reported data acquired by quantitation of P700, cytochrome b-559 and/or molecular oxygen by flash illumination in the same species
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