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Abstract
The paper describes a system of colorimetric analysis of opaque liquid samples performed with a new instrument known as an opaque sample analyzer (OSA). Alkaline phosphatase, peroxidase, and β-glucosidase were assayed with the OSA in two opaque liquid substrates, cow's milk and microbiologically debittered olive brine. The nondestructive OSA analysis of heat-treated and untreated milk samples permitted quantitative evaluation of the reaction products of alkaline phosphatase and peroxidase with a sensitivity clearly better than that of the qualitative methods currently employed in routine analyses. The olive brine analysis with the OSA allowed the demonstration, for the first time in this matrix, of the activity of β-glucosidase produced by Lactobacillus plantarum used as the inoculum to debitter the table olives.
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