Abstract

The aim of the present study was to evaluate the microbiological quality of raw milk from milk vending machine and heat treated milk during storage. There were analyzed 120 samples of milk (30 samples of fresh milk, 30 samples of raw milk stored 4 day at 4 °C, 30 samples of heat treated milk - 70 °C stored 4 day at 4 °C and 30 samples of heat treated milk - 100 °C stored 4 day at 4 °C). Total viable counts (TVC), coliform bacteria (CB) and microscopic filamentous fungi (MFF) were determined by microbiological analysis. Plate dilution method were used for microbiological analysis. The number of total viable counts (TVC) in fresh milk ranged from 4.08 log KTJ.mL-1 to 4.89 CFU.mL-1. TVC in raw milk after storage ranged from 5.31 log CFU.mL-1 to 6.81 log CFU.mL-1. TVC in heat treated milk with temperature 70 °C after storage ranged from 3.89 log CFU.mL-1 to 4.45 log CFU.mL-1 and TVC in heat treated milk with temperature 100 °C after storage ranged from 2.96 log KTJ.mL-1 to 3.91 log KTJ.mL-1 in heat treated milk with temperature 100 °C after storage. The number of CB were in range from 1.49 log CFU.mL-1 to 1.89 log CFU.mL-1 in fresh milk, from 1.99 log CFU.mL-1 to 2.61 log CFU.mL-1 in raw stored milk. Coliform bacteria were not present in heat-treated milk samples. The values of MFF ranged from 0 log CFU.mL-1 to 2.01 log CFU.mL-1 in fresh milk, from 1.43 log CFU.mL-1 to 3.98 log CFU.mL-1 in raw milk after storage, from 1.33 log CFU.mL-1 to 3.41 log CFU.mL-1 in heat treated milk with temperature 70 °C after storage and from 1.30 log CFU.mL-1 to 3.32 log CFU.mL-1 in heat treated milk with temperature 100 °C after storage.

Highlights

  • Milk and dairy products are important components of the diet worldwide

  • The quality and shelf life of liquid milk as well as dairy products are often compromised by flavor, odors, and visual defects arising from the bacterial growth and activities of heat-stable enzymes produced by psychrotrophic bacteria before processing (Techer et al, 2014)

  • The number of total viable counts (TVC) ranged from 4.08 log CFU.mL-1 to 4.89 CFU.mL-1 in fresh milk, from 5.31 log CFU.mL-1 to 6.81 log CFU.mL-1 in raw milk after storage, from 3.89 log CFU.mL-1 to 4.45 log CFU.mL-1 in heat treated milk with temperature 70 °C after storage and from 2.96 log CFU.mL-1 to 3.91 log CFU.mL-1 in heat treated milk with temperature 100 °C after storage (Figure 1)

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Summary

Introduction

Milk and dairy products are important components of the diet worldwide. The quality and shelf life of liquid milk as well as dairy products are often compromised by flavor, odors, and visual defects arising from the bacterial growth and activities of heat-stable enzymes produced by psychrotrophic bacteria before processing (Techer et al, 2014).Milk testing and quality control should be carried out at all stages of the dairy chain (Zajác et al, 2015).The quality and safety of raw cow’s milk is very important for dairy companies and consumers of milk products. Milk and dairy products are important components of the diet worldwide. The quality and shelf life of liquid milk as well as dairy products are often compromised by flavor, odors, and visual defects arising from the bacterial growth and activities of heat-stable enzymes produced by psychrotrophic bacteria before processing (Techer et al, 2014). The quality and safety of raw cow’s milk is very important for dairy companies and consumers of milk products. Due to its high nutritional value together with the neutral pH and high water activity, raw milk is good growth medium for different micro-organisms, whose multiplication depends mainly on temperature and on competing micro-organisms and their metabolic products. In order to guarantee its microbial safety and to prolong its shelf-life, milk is heat treated (Claeys et al, 2013)

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