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Abstract
The aim of this study was to apply high performance liquid chromatography – UV detector to determine desmopressin in pharmaceutical preparation using one pump (isocratic pump) and ODS, 5um column. And Mobil phase acetonitrile: N, N diethyl formamide in ratio of 85:15 which show good quantitative and qualitative analysis in desmopressin acetate nasal spray (Cipla LTD INDIA) New controlled condition for setting-up the system to get a result with high resolution was shown in this study. Keywordsdesmopressin, HPLC method , isocratic pump and system set-up
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