HPLC Analysis of Patulitrin and Patuletin in Flowers of Tagetes Patula L.

Abstract

The flowers of spreading marigold (Tagetes patula L.) are new interesting view of medicinal plant material. This raw material is one of the valuable sources of flavonoids. The aim of this study was to develop a method for the quantitative determination of the patulitrin (7-O-β-D-glucopyranoside of 3,5,7,3’,4’-pentahydroxy-6-methoxyflavone) and patuletin (3,5,7,3’,4’-pentahydroxy-6-methoxyflavone) content in flowers of spreading marigold by HPLC. The inflorescences of spreading marigold (Tagetes patula L.) variety "Tangerine", collected in August-September 2018 and 2019 in the Botanical Garden of the Samara University. The water-alcohol extracts were obtained from spreading marigold flowers, which were used for qualitative and quantitative analysis, as well as for preparative isolation of individual compounds. There was developed the method of determination of patulitrin and patuletin in the spreading marigold flowers (Tagetes patula L.) using high performance liquid chromatography (HPLC). The content of dominant flavonoid patulitrin in the flowers of T. patula varies from 5.11±0.18% to 5.64±0.17%. The error of single determination of patulitrin in the spreading marigold flowers with confidence probability of 95 % is ±3.32%. The results of the studies carried out indicate the advisability of the standardization of the flowers of the spreading marigold by determining of the content of the main and diagnostically significant flavonoid, patulitrin, using the HPLC method and detection on a UV detector at a wavelength of 360 nm.