大豆胚軸經Aspergillus niger M46固態發酵後評估其生理活性之探討

Abstract

The purpose of the study was to determine the contents of polyphenols and flavonoids in the extracts of the fermented soybean hypocotyl in solid-state fermentation by Aspergillus niger M46, and evaluating their physiological properties. In this study, 95%ethanol, 99.9%methanol and 70% methanol were used as the solvent to extract the fermented soybean hypocotyl. The results showed that the DPPH radical scavenging ability was 42.58, 44.93 and 56.28%, respectively, at the extract concentration of 0.53 mg/mL. Significantly higher (p<0.05) of DPPH scavenging ability of 70% methanol extracts was found when compared to the other two solvents. After solid-state fermentation, the total polyphenolic content of fermented hypocotyl was approximately 3 fold higher than non-fermented samples. The content of flavonoids in fermented hypocotyl was 3.9 fold higher than non-fermented ones. The DPPH radical scavenging ability and reducing power of fermented sample was 6.49 and 8.12 fold higher than non-fermented hypocotyl, respectively. The growth of cancer cell was inhibited at 81.53 and 51.47% when treated with 1.3333 mg/mL for AGS and Caco-2 cells, respectively. However, hypocotyl without fermentation had no inhibition effect on those cancer cell. Regarding anti-inflammatory activity, methanol extracts of fermented and non-fermented hypocotyl could suppress NO production in LPS-induced macrophages, and fermented sample showed 1.61 fold higher than non-fermented hypocotyl. In addition to suppression of NO production, fermented hypocotyl extracts could suppress IL-1b production significantly; however, for suppression of IL-6 and TNF-a production in LPS-induced macrophages, significant effect was observed only at extract concentration of 400 ug/mL. For 3T3-L1 preadipocytes, the hypocotyl extract had no suppressive effect on their differentiation, but high dose could cause the death of the cells. Methanol extracts of fermented hypocotyl were chromatographed and separated using HPLC. Fractions at retention times of 23, 25, 34 and 35 min. were collected to evaluation their functionality. The results showed that the fraction at 34 min. retention time had the best DPPH radical scavenging ability and NO production suppression ability. The fraction of fermented hypocotyl at 34 min. retention time showed potential to be the functional ingredient and further studies are needed.