Abstract
Male albino rats were castrated and their ascorbic acid, dehydroascorbic acid, and diketogulonic acid were determined in liver and urine, while only the total ascorbic acid was determined in blood following 2 and 6 wk after castration. Activities of ascorbic-acid-degrading enzymes dehydroascorbatase and 2,3-diketoaldonate decarboxylase were studied in the liver and kidney. In castrated rats there was an appreciable decrease in the ascorbic acid and dehydroascorbic acid contents of the liver. On the other hand, there was a significant increase in the diketogulonic acid content of liver. The contents of ascorbic acid and diketogulonic acid in the urine of castrated rats decreased significantly, while there was an increase in the diketogulonic acid content. There was also a slight decrease in the total ascorbic acid content of blood. An appreciable increase in the activity of dehydroascorbatase with no change in the activity of 2,3-diketoaldonate decarboxylase was noticed in the liver and kidney of these animals. All corresponding alterations were more significant in rats 6 wk after castration.
Published Version
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