Abstract
Laccase was immobilized on an electrode modified with a cysteine self-assembled monolayer and coated with functionalized quantum dots. The immobilized laccase is capable of directly transferring an electron. Immobilized laccase retained its activity to oxidize ascorbic acid (AA), and the apparent Michaelis–Menten constant was found to be 0.47 mM. The modified electrode was used to determine AA in the 10 to 140 μM concentration range, with linear response curve and a detection limit of 1.4 μM (s/n = 3).
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