Abstract
Background: Finding effective dose is the most crucial step before commencing any mutagen treatment. The present study determined the lethal median dose in two mungbean genotypes, ‘Pusa 1031’ and ‘Pusa 1431’, using two different chemical mutagens namely Ethyl Methane Sulphonate and Sodium Azide. Methods: A pair of mungbean genotypes “Pusa 1031” and “Pusa 1431” were subjected to varying concentrations of two chemical mutagens i.e., Ethyl Methane Sulphonate (EMS) (10-100 mm with a ten mm different) and Sodium Azide (SA) (0.01 mm - 0.05 mm) followed by germinating the seeds in trays containing soil rite. Result: EMS treatment in two genotypes displayed LD50 values as 58.81 mM and 45.04 mM for the genotypes ‘Pusa 1031' and ‘Pusa 1431’, respectively. Lethal dose 50 was determined as 0.047 mM for both genotypes when treated with sodium azide. Seedling characters exhibited a linear response with dose augmentation for both the chemical mutagens, despite displaying similar LD50 values both genotypes exhibited remarkable differences in seedling parameters when treated with sodium azide.
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