Arylazido aminopropionyl ATP, an active site directed photoaffinity reagent for mitochondrial adenosine triphosphatase

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The photoaffinity label, arylazido aminopropionyl ATP ∗ ∗ Abbreviations: arylazido aminopropionyl ATP; (3′-0-{3-[N-(4-azido-2-nitrophenyl)amino]propionyl}adenosine-5′-triphosphate). , brings about a photodependent inhibition of mitochondrial F 1-ATPase and an associated specific covalent labeling of the soluble enzyme. In addition the ATP analog can act as a substrate when incubated with F 1-ATPase in the dark.