Abstract
Naturally, seaweed has a high arsenic content due to the arsenic bioaccumulation process. Until now, research on arsenic in seaweed has reached the stage of identifying the types of arsenic. One of the critical points in the arsenic speciation method in seaweed is the arsenic extraction stage. The extraction process of arsenic in seaweed is influenced by several factors, including the time of sonication exposure and the number of treatments in the extraction process. This process will be different for each type of seaweed due to differences in cell wall composition. This research is an early stage in conducting arsenic speciation. The purpose of this study was to determine the time of sonication exposure and the number of treatments that were most efficient in extracting arsenic in seaweed species Eucheuma cottoni, Eucheuma spinosum, and Gracilaria sp. In the number of treatments, the extraction process was carried out with five treatment levels (1, 2, 3, 4, and 5), with the maximum extraction percent value was obtained in the fifth treatment for the three types of seaweed. The sonication time factor was carried out at three treatment levels (5, 10, 15 minutes), with the most optimal extraction percent value was obtained at 10 minutes for E. spinosum and Gracilaria sp. Meanwhile, E. cottoni got the most optimal time at the third level (15 minutes). Under these conditions, the extraction process resulted in the percentage of arsenic extraction of 32.58% (Gracilaria sp.), 44.54% (E. spinosum), and 46.41% (E. cottoni).
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