Arsenic mobilization in nZVI residue by Alkaliphilus sp. IMB: Comparison between static and flowing incubation

Abstract

Arsenate reducing bacteria (AsRB) enhance arsenic (As) release via reducing As(V) to As(III), and As mobility is usually controlled by As(III) re-uptake on in-situ formed secondary iron minerals. The re-uptake of As(III) under groundwater flow conditions significantly impacts the fate and transport of As. Herein, a novel As(V)-reducing bacterium Alkaliphilus IMB was isolated in an As-contaminated soil. Scanning transmission X-ray microscopy showed that dissolved As(V) was mainly bound to the cell walls whereas dissolved As(III) was homogeneously distributed around IMB, indicating that As(V) reduction occurs outside the cell membrane. To explore the effect of IMB on As mobility, IMB was incubated with As-loaded nanoscale zero-valent iron (nZVI) residues under static and flowing conditions. IMB reduced 100% dissolved As(V) to As(III) even in a short contact time (∼1 h) during flowing incubation. The formation of As(III) did not influence As mobility under static condition as evidenced by the comparable concentrations of released As in the presence of IMB (8.5% to total As) and the abiotic control (10% to total As). Biogenic As(III) was re-adsorbed on the solids as shown by the higher ratio of solid-bound As(III) to total As in the presence of IMB (54%) than that in the abiotic control (12%). By contrast, the degree of As(III) re-adsorption was inhibited in the flowing environment, as suggested by the lower As(III) ratio in the solid (31%). This inhibition can be ascribed to the relatively slow adsorption of As(III) compared with the quick reduction of As(V) (∼1 h). Thus, IMB significantly enhanced As release during flowing incubation as shown that 9.8% As was released in the presence of IMB while 2.1% As in the abiotic control. This study found the contrary effect of AsRB on As mobility in static and flowing environments, highlighting the importance of re-adsorption rate of As(III).