Arsenic methylation behavior and microbial regulation mechanisms in landfill leachate saturated zones

Abstract

Arsenic (As) is a potential contaminant in landfill. As methylation has been considered as a detoxification mechanism to address this problem. In this study, microcosm incubation was used to simulate leachate saturation zone (LSZ) and other landfill zones scenarios to explore the As methylation behavior. The As methylation rate of LSZ is 11.75%, which is slightly higher than that of other zone of landfill (10.87%). However, the difference was greatly increased by the addition of moderate content of As(III), with values of 29.25% in LSZ and 4.61% in other zones. The microbial community structure varied greatly between zones and a higher abundance of arsM was observed in the LSZ, which enhanced As methylation. Based on the annotated As functional genes from the KEGG database, the microbial As methylated pathway was summarized. Higher relative abundances of gst and arsC promoted the formation of more trivalent As substrates, stimulating the methylation behavior for As detoxification in the LSZ. According to microbial arsM contribution analysis, unclassified_p__Gemmatimonadetes, unclassified_p__Actinobacteria, unclassified_o_Hydrogenophilales, and Intrasporangium were the primary As methylation bacteria in the LSZ, while unclassified_f__Chitinophagaceae and unclassified_c_Gammaproteobacteria were the primary contributors in other landfill zones. These results highlight the specific As methylation process in the LSZ, and these insights could improve the control of As contamination in landfill sites.