Abstract
Summary Strictly anaerobic mollicutes (mycoplasmas) of the genus Anaeroplasma were examined for enzymic activities of aromatic amino acid and carbohydrate metabolism. Anaeroplasma intermedium and Anaeroplasma varium possessed activities for four enzymes associated with aromatic amino acid biosynthesis: 7~phospho-2-dehydro-3-deoxy-D-arabino-heptonate synthase, shikimate dehydrogenase, prephenate dehydrogenase, and prephenate dehydratase. Shikimate dehydrogenase was detected with NAD+ but not NADP+. The effects of potential allosteric effectors on the activities of three of these enzymes were tested. Furthermore, Anaeroplasma bactoclasticum, Anaeroplasma varium, and Anaeroplasma abactoclasticum had enzymic activities of the Embden-Meyerhof-Parnas pathway for glycolytic catabolism including PPi dependent phosphofructokinase. Anaeroplasma bactoclasticum and Anaeroplasma varium also possessed isocitrate dehydrogenase, an enzyme not detected in other mollicutes. In addition, FAD+-linked pyruvate synthase was detected in Anaeroplasma bactoclasticum and Anaeroplasma varium; this enzyme has not been previously reported to occur in mollicutes.
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