Abstract
A method for the separation by starch gel and the in situ detection of the isozymes of arginase is presented. The method is capable of detecting 0.2 units of activity; it is specific, giving no staining in the absence of either arginine or urease from the developing solution. For purposes of the present study all parameters have been optimized for the separation and localization of arginase isozymes in tissue extracts. The method ought to be applicable, however, with appropriate modifications, to other enzymes yielding either alkaline reaction products (detectable in the presence of excess thiol) or yielding free thiol (such as reduced Coenzyme A) in alkaline medium.
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