Abstract
AbstractA method for quantitative analysis of neutral lipids has been developed. Four different techniques have been combined for this purpose—supercritical fluid chromatography (SFC), silver ion chromatography, packed microcolumns and miniaturized evaporative light‐scattering detection (ELSD). The development and optimization of the method are discussed. The separation of a series of vegetable, fish and hydrogenated oils was demonstrated. Application of eluent composition programming resulted in excellent separation of complex samples. Packed microcolumn argentation SFC provides at least as high a separation power as corresponding high‐performance liquid chromatography methods. The combination of packed microcolumn SFC and miniaturized ELSD constitutes a powerful analytical system for the quantitative analysis of triacylglycerols.
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