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Abstract
Manganese content and the amount of oxygen-evolving centres have been determined for purified PS II complexes from the cyanobacterium Synechococcus sp. by atomic absorption and measurements of flash-induced oxygen yields, respectively. A stoichiometry of six manganese per oxygen-evolving centre was observed. This was found not only for PS II preparations with different activities of oxygen evolution, but also when deactivating treatments by low-salt or NH 2 OH incubation were carried out. Our results call into question the current thinking that four Mn ions are located in the water-oxidizing complex. A hexanuclear manganese model is discussed, instead.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Reviews on Bioenergetics
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