Arctium lappa Extract Suppresses Inflammation and Inhibits Melanoma Progression.

Bruno A C Nascimento,Mirella R A Moreira,Marília G Gallucci,Lúcia H Faccioli,Inaê M G Silveira,Cristiane Tefé-Silva,Karina F Zoccal,Alyne F G Meirelles,Luiz G Gardinassi,Mariana A Tomé,Júlia Fernanda D Oliveira

doi:10.3390/medicines6030081

Bruno A C Nascimento, Mirella R A Moreira + Show 9 more

Open Access

https://doi.org/10.3390/medicines6030081

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Abstract

Background: Arctium lappa has been used as popular medicinal herb and health supplement in Chinese societies. Bioactive components from A. lappa have attracted the attention of researchers due to their promising therapeutic effects. In this study, we investigated the effects of A. lappa hydroalcoholic extract (Alhe) during different models of inflammation, in vivo. Methods: The anti-inflammatory activity was evaluated through the air pouch model. For this, mice received an inflammatory stimulus with lipopolysaccharide (LPS) and were later injected with Alhe. To assess anti-tumoral activity, the animals were inoculated with B16F10 cells and injected with Alhe every 5 days, along the course of 30 days. Controls were submitted to the same conditions and injected with the vehicle. Peritoneal or air pouch fluids were collected to evaluate leukocyte counting or cellular activation via quantification of cytokines and nitric oxide. Results: Alhe injection reduced the neutrophil influx and production of inflammatory mediators in inflammatory foci after LPS or tumor challenges. Furthermore, Alhe injection reduced tumor growth and enhanced mice survival. Conclusions: Collectively, these data suggest that Alhe regulates immune cell migration and activation, which correlates with favorable outcome in mouse models of acute inflammation and melanoma progression.

Highlights

Inflammation is a complex process operating in response to noxious stimuli and tissue damage.Acute inflammation is characterized by tissue infiltration and accumulation of leukocytes, such as neutrophils and monocytes, and increasing vascular permeability that promotes protein extravasation and edema formation [1,2]
We investigated the effects of A. lappa hydroalcoholic extract (Alhe) injection on different models of inflammation, in vivo
We confirmed that A. lappa exhibits robust anti-inflammatory properties in different modalities of inflammation

Summary

Introduction

Inflammation is a complex process operating in response to noxious stimuli and tissue damage.Acute inflammation is characterized by tissue infiltration and accumulation of leukocytes, such as neutrophils and monocytes, and increasing vascular permeability that promotes protein extravasation and edema formation [1,2]. Inflammation is a complex process operating in response to noxious stimuli and tissue damage. Excessive or unbalanced inflammation might lead to tissue damage and become counterproductive. Skin cancer is characterized by an imbalance towards reduced apoptosis or increased cell proliferation and survival in the epidermis [4,5], combined with a failure of the immune system to contain the tumoral development. A. lappa hydroalcoholic extract (Alhe) during different models of inflammation, in vivo. Methods: The anti-inflammatory activity was evaluated through the air pouch model. Mice received an inflammatory stimulus with lipopolysaccharide (LPS) and were later injected with Alhe. To assess anti-tumoral activity, the animals were inoculated with B16F10 cells and injected with Alhe every

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Conclusion