Arctiin blocks hydrogen peroxide-induced senescence and cell death though microRNA expression changes in human dermal papilla cells.

Seunghee Bae,In-Sook An,Sungkwan An,Ho Jung Jung,Kyu Joong Ahn,Kyung Mi Lim,Kwang Sik Lee,Hwa Jun Cha,Ghang Tai Lee,Jeong Pyo Lee,Kun Kook Lee

doi:10.1186/0717-6287-47-50

Seunghee Bae, In-Sook An + Show 9 more

Open Access

https://doi.org/10.1186/0717-6287-47-50

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Journal: Biological research	Publication Date: Sep 30, 2014
Citations: 58	License type: cc-by

Affiliation: Konkuk University, Cosmetics Europe

Abstract

BackgroundAccumulating evidence indicates that reactive oxygen species (ROS) are an important etiological factor for the induction of dermal papilla cell senescence and hair loss, which is also known alopecia. Arctiin is an active lignin isolated from Arctium lappa and has anti-inflammation, anti-microbial, and anti-carcinogenic effects. In the present study, we found that arctiin exerts anti-oxidative effects on human hair dermal papilla cells (HHDPCs).ResultsTo better understand the mechanism, we analyzed the level of hydrogen peroxide (H2O2)-induced cytotoxicity, cell death, ROS production and senescence after arctiin pretreatment of HHDPCs. The results showed that arctiin pretreatment significantly inhibited the H2O2-induced reduction in cell viability. Moreover, H2O2-induced sub-G1 phase accumulation and G2 cell cycle arrest were also downregulated by arctiin pretreatment. Interestingly, the increase in intracellular ROS mediated by H2O2 was drastically decreased in HHDPCs cultured in the presence of arctiin. This effect was confirmed by senescence associated-beta galactosidase (SA-β-gal) assay results; we found that arctiin pretreatment impaired H2O2-induced senescence in HHDPCs. Using microRNA (miRNA) microarray and bioinformatic analysis, we showed that this anti-oxidative effect of arctiin in HHDPCs was related with mitogen-activated protein kinase (MAPK) and Wnt signaling pathways.ConclusionsTaken together, our data suggest that arctiin has a protective effect on ROS-induced cell dysfunction in HHDPCs and may therefore be useful for alopecia prevention and treatment strategies.

Highlights

Accumulating evidence indicates that reactive oxygen species (ROS) are an important etiological factor for the induction of dermal papilla cell senescence and hair loss, which is known alopecia
We studied the biological events of human hair dermal papilla cells (HHDPCs) in the presence of arctiin and/or H2O2 and performed cell-based assays to determine whether H2O2-induced cell dysfunction could be inhibited by arctiin treatment
Arctiin inhibits H2O2-mediated cell proliferation loss in a dose-dependent manner in HHDPCs Before we analyzed the inhibitory effect of arctiin against H2O2-induced cell dysfunction, we preferentially sought to evaluate the effect of arctiin on HHDPC growth

Summary

Introduction

Accumulating evidence indicates that reactive oxygen species (ROS) are an important etiological factor for the induction of dermal papilla cell senescence and hair loss, which is known alopecia. Under stressful environmental conditions (e.g., ionizing radiation, ultraviolet (UV) radiation, drugs, and smoke), the levels of ROS, including hydrogen peroxide (H2O2), are significantly increased [2]. These ROS subsequently activate various signaling molecules, including p53 and mitogen-activated protein kinase (MAPK) to induce cell growth arrest and apoptosis [3,4]. Alopecia is a hair loss skin disorder that typically causes baldness [10] It was originally defined as an age-dependent or androgen-dependent mechanism in dermal papilla cells (DPCs) [11]. These finding have led to the hypothesis that ROS may be an important target when designing therapeutic strategies to prevent or treat alopecia

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