Effect of PDGF-C on biological characters of human dermal papilla cells in vitro

Abstract

Objective To determine the effect of platelet-derived growth factor-C (PDGF-C) on biological characters of human hair dermal papilla cells cultured in vitro. Methods The human dermal papilla cells(HDPCs) were isolated from human hair skin obtained from rhytidectomy procedure and then cultured in vitro. Cell counting and CCK-8 assay were used to detect the effects of PDGF-C (0, 10, 20, 30 and 40ng/ml) on the proliferation of HDPCs at 0, 1d, 2d, 3d, 4d, 5d, 6d. Under the optimal concentration, flow cytometry was used to detect cell phases. Transwell assay and cell scratch test were performed to detect cell migration. Alkaline Phosphatase Activity Assay kit was used to detect the inductive activity of HDPCs. Results PDGF-C significantly induced the proliferation of HDPCs. PDGF-C of 30ng/mL promoted the HDPCs proliferation at a summit and increased the percentage of the cells arrested at S phase (P<0.05). PDGF-C also increased the migration populations of cultured HDPCs. The cell number in lower side of the transwell insert membrane of 30ng/ml PDGF-C treated group was (361.3 ± 24.95)while the control was(246.8 ± 7.525), showing significant difference (P<0.05). The alkaline phosphate activity of cultured HDPCs was increased comparing to the control group, the difference was significant (P<0.05). Conclusions PDGF-C can promote the proliferation, migration and inductive activity of cultured human dermal papilla cells, which might be beneficial to promote the cultivation of human dermal papilla cells in vitro. Key words: Platelet-derived growth factor-C; Dermal papilla cells; Cell proliferation; Cell movement