Arbuscular mycorrhizal fungal hyphae contribute to the uptake of polycyclic aromatic hydrocarbons by plant roots

Abstract

The arbuscular mycorrhizal (AM) hyphae–mediated uptake of polycyclic aromatic hydrocarbons (PAHs) by the roots of ryegrass ( Lolium multiflorum Lam.) was investigated using three-compartment systems. Glomus mosseae and Glomus etunicatum were chosen, and fluorene and phenanthrene were used as representative PAHs. When roots were grown in un-spiked soils, AM hyphae extended into PAH-spiked soil and clearly absorbed and transported PAHs to roots, resulting in high concentrations of fluorene and phenanthrene in roots. This was further confirmed by the batch equilibration experiment, which revealed that the partition coefficients ( K d) of tested PAHs by mycorrhizal hyphae were 270–356% greater than those by roots, suggesting the great potential of hyphae to absorb PAHs. Because of fluorene’s lower molecular weight and higher water solubility, its translocation by hyphae was greater than that of phenanthrene. These results provide new perspectives on the AM hyphae–mediated uptake by plants of organic contaminants from soil.