Arabica Coffee (Coffea Arabica) Fruit Skin Potential Towards the Increase of Fibroblast Cells Amount Within Socket Post Tooth Extraction of Male Wistar Mouse

Abstract

Background: Tooth extraction can cause wound in hard tissue and soft tissue inside oral cavity. Wound healing process involves cell proliferation especially fibroblast cells. To accelerate fibroblast cell proliferation alternative substance that can accelerates wound healing process is needed which is arabika coffee fruit skin. Arabika coffee fruit skin contains substances such as polyphenols, tannin, chlorogenic acid, and caffeine expected to be able to stimulate fibroblast cells amount. Objective: To understand arabika coffee fruit skin potential towards increase in number of fibroblast cells on the socket after tooth extraction. Method: Research plan post test only control group design with samples of 24 wistar mouse. Samples used grouped into two groups which are control and treatment group. Then treatment is given in 3 days, 5 days, and 7 days. Then they are decapitated to undergo tissue processing with HE coloring and post tooth extraction socket fibroblast cells counting. Acquired data then analyzed using One Way ANOVA and LSD test. Result: fibroblast cell amount having significant raise (p<0,05) in treatment group compared to control group. Conclusion: Arabika coffee fruit skin potentially increase fibroblast cells amount in socket post tooth extraction of male wistar mouse. Keywords: Tooth extraction, wound healing, fibroblast cells.