Abstract
ABSTRACT Flow cytometry (FC) is an essential tool for diagnosis, prognosis and therapeutic follow-up of several hematologic malignancies. In addition, it performs the quantification of lymphocytes subpopulations for diagnosis and monitoring of primary and acquired immunodeficiencies through the antigenic expressions of CD19 and CD20 for B lymphocytes; CD2, CD3, CD4, CD8 for T lymphocytes; and CD56 and CD16 for the identification of natural killer (NK) cells. The cytometry technique has revolutionized the way that the cells are identified, and over the years this platform has progressed with several advances in hardware and software that aim to improve workflow resulting in higher productivity, quality and cost savings. The Aquios CL - Beckman Coulter (BC) is an example of this advance because it is a complete automation instrument in flow cytometry called “Load & Go flow cytometer” for quantification of lymphocyte subpopulations in the routine diagnosis. In this study, the Aquios CL was validated, and quantification in frequency and absolute numbers of the lymphocyte subpopulations had an excellent correlation with the results obtained by the dual platform quantification performed in the Cytomics FC500 (BC) and automated Sysmex XE-2100 cell analyzer.
Highlights
Flow cytometry (FC) is an essential tool for diagnosis, prognosis and therapeutic follow-up of several hematological malignancies, as well as for primary or acquired immunodeficiencies[1,2,3].Immunophenotyping by FC quantifies lymphocyte populations and subpopulations, evaluating, basically, antigenic expressions of CD19 and CD20 for B lymphocytes; CD2, CD3, CD4 and CD8 for T lymphocytes; and CD56 and CD16 for identification of natural killer (NK) cells[2, 4]
The search for services equipped with the technology of CD4 T lymphocyte quantification has become more and more frequent, and the use of tools that add speed, efficacy and quality to diagnosis and to monitoring of immunodeficiencies has been made essential in clinical practice[8, 10]
The comparison between the different platforms of lymphocyte quantification took into consideration results in frequency and absolute numbers and the final test interpretation
Summary
Immunophenotyping by FC quantifies lymphocyte populations and subpopulations, evaluating, basically, antigenic expressions of CD19 and CD20 for B lymphocytes; CD2, CD3, CD4 and CD8 for T lymphocytes; and CD56 and CD16 for identification of natural killer (NK) cells[2, 4]. Quantification of these populations helps monitor immunosuppressing and immunomodulatory therapies and the diagnosis and monitoring of primary immunodeficiencies, besides contributing to the evaluation of post-transplant lymphocytic populations[4,5,6]. The search for services equipped with the technology of CD4 T lymphocyte quantification has become more and more frequent, and the use of tools that add speed, efficacy and quality to diagnosis and to monitoring of immunodeficiencies has been made essential in clinical practice[8, 10]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
