Abstract
Total lignans were extracted using an aqueous two-phase system combined with an ultrasound-assisted extraction method from Schisandra chinensis Baill (SCB) seeds. The three main functional components, schizandrin (SA), schisantherin A (SAA) and deoxyschizandrin (DSA), were selected as markers. Initially, the phase separation ability of various salts and ethanol were compared, then response surface methodology (RSM) experiments were used to investigate the effects of extraction time, ultrasound power and solvent to solid ratio on extraction efficiency. The partition coefficient and recovery of three markers indicated that a system based on ethanol and (NH4)2SO4 was the best system. The optimal phase composition was 25% (w/w) (NH4)2SO4 and 19% (w/w) ethanol, and the partition coefficients of SA, SAA and DSA were 77.09, 76.73 and 86.99, respectively, with a recovery of >93%. The optimal conditions for total lignan extraction was selected as 20:1 (solvent:solid), 800W and 61.1min, which resulted in yields for SA, SAA and DSA of 13.10, 1.87 and 1.84mg/g, respectively. Compared with an 80% ethanol (v/v) extraction method, this method has the advantages of combining extraction with purification in a single step and yields higher purity total lignans from the seeds of SCB.
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