Aqueous levels of protein and IL-6 in silicone oil and C3F8 tamponade rabbit eyes

Abstract

Objective To evaluate the aqueous protein and IL-6 levels induced by cryotherapy in rabbit eyes filled with silicone oil and C3F8.Methods The rabbit eyes were filled with silicone oil,C3F8 or BSS (control group) after vitrectomy and cryotherapy.Protein and IL-6 levels were examined by ELISA and BCA on post-op day 14 and 28.Results The IL-6 levels in silicone oil group (647.71±87.26) pg/ml,(392.98±32.4)pg/ml were significantly higher than those in C3F8 (523.46±43.02)pg/ml,(85.47±14.03)pg/ml and control group (72.43±13.9)pg/ml,(72.43±13.9)pg/ml on post-op day 14 and 28 (P ＜0.05).The protein levels in silicone oil and gas group showed no significant difference on post-op day14.The protein levels in silicone oil (6.27±0.88)mg/ml and gas group (2.80±0.85)mg/ml showed statistically significant difference on post-op day 28 (P ＜0.05).Conclusions Silicone oil tamponade delays BRB repair in rabbit eyes with cryotherapy. Key words: Silicone oil; Cryotherapy; Blood retinal barrier; IL-6