Aquaporin-1: An Osmoinducible Water Channel in Cultured mIMCD-3 Cells

Abstract

The expressions of aquaporin-1 (AQP-1) in cultured mIMCD-3 cells were studied. There was no detectable AQP-1 in cells grown in serum-containing growth medium (SM, 297 ± 2 mOsm/kg·H2O). When SM was supplemented with NaCl (406 ±2 mOsm/kg·H2O), cellular AQP-1 was induced. A further increase in medium osmolarity with NaCl (493 ± 3 mOsm/kg·H2O) had conferred cells an 2.5 to 3-fold increase of AQP-1 expression over those grown in the 406 ± 2 mOsm/kg·H2O medium. Moreover, AQP-1 was found to be translocated from cytosol to membrane. In addition, exposing the mIMCD-3 cells to vasopressin (AVP, 10−8M) and/or NaCl-supplemented serum-free media (496 ± 3 mOsm/kg·H2O) for 6h did not render them to produce AQP-1. However, AQP-1 was induced after 24h of incubation, with an 1.5-fold additive effect by AVP. Our RT-PCR data had confirmed the NaCl inducibility and AVP synergism in AQP-1 expression at both mRNA and protein levels. This suggests a new role for cellular AQP-1 and AVP in overcoming osmotic stress in anin vitrosystem.