Abstract
The impedimetric detection of a protein, lysozyme (LYS), was carried out herein by aptamer-immobilized single-use pencil graphite electrodes (PGEs). The aptamer was immobilized onto electrochemically activated surface of electrode without using any chemical agents, or any types of nanomaterials. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques were applied to analyze the electrochemical behavior of unmodified PGE and aptamer immobilized PGE. The interaction of aptamer withits target protein, LYS, was then investigated by EIS. The limit of detection for LYS was found to be 1.44μg/mL (equals to 100.65nM). The developed aptasensor specific to LYS presented high selectivity against to bovine serum albuminand thrombin.
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