Applying a sandwich-like strategy for dual 'light up' capture and eradication of Staphylococcus aureus using magnetically functionalized materials

Abstract

In this study, we proposed an innovative application of porcine immunoglobulin G (IgG)-functionalized Fe3O4 (IgG-Fe3O4) specifically designed to target and capture Staphylococcus aureus (S. aureus). In addition, aminophenylboronic acid-modified tetraphenylethylene nanoparticles (APBA-TPE NPs) were utilized, establishing a sandwich-type dual recognition system via interactions with the bacteria's extracellular glycolipids. This approach enables highly sensitive and precise detection of bacterial presence, with a limit of detection (LOD) reaching down to 5.0 CFU/mL. Specifically, the prepared APBA-TPE NPs achieved 99.99% bacterial inactivation within 60 min at a concentration of 200 µg/mL. The results showed that APBA-TPE NPs possess a remarkable capacity for reactive oxygen species (ROS) production, which could attack the bacterial cell membrane, leading to bacterial lysis and content leakage, and ultimately to bacterial death. Furthermore, the material still showed good recoveries ranging from 88.5% to 93.5% in actual water samples, as well as a favorable sterilizing effect of killing all microorganisms for 60 min. This research provides new strategies and insights into the construction of methods for the specific capture, detection, and inactivation of S. aureus.