Abstract
The applications of freeze-fracture cytochemistry to the study of tissues and cells are described. Two approaches will be presented: label-fracture and fracture-label. In both techniques, colloidal gold probe is used as the marker system. The use of cytochemistry in combination with freeze-fracture for the study of distribution of receptor sites, antigens and glyco- conjugates on freeze-fractured plasma membranes as well as on fractured intracellular organelles is illustrated respectively by the two models described below. A variant of the fracture-label technique, Backscattered Electron Imaging of labeled tissues following freeze-fracture cytochemistry, is also introduced as a novel method for the 3-dimensional visualization of the in situ distribution of receptor sites on freeze-fractured membranes.1.Molecular demarcation of surface domains in boar spermatozoa as visualized by label-fracture cytochemistry.The label-fracture technique has been previously described. In brief, boar sperm cells are fixed, labeled and impregnated with glycerol.
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